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Clin Oral Implants Res. 2016 Nov;27(11):1360-1367. doi: 10.1111/clr.12744. Epub 2016 Jan 8.

Effect of poly (lactide-co-glycolide) (PLGA)-coated beta-tricalcium phosphate on the healing of rat calvarial bone defects: a comparative study with pure-phase beta-tricalcium phosphate.

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Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.



To investigate the effect of poly (lactide-co-glycolide) (PLGA)-coated β-tricalcium phosphate (TCP) as a scaffold on bone regeneration in rat calvaria.


Bilateral critical-sized defects were created in the calvaria of 20 Sprague Dawley rats. Defects of each rat were filled with pure-phase β-TCP or PLGA/β-TCP, or left as unfilled control. The healing was evaluated by micro-computed tomography, histological, and immunohistochemical analyses. Tartrate-resistant acid phosphatase (TRAP) staining was also performed to assess the resorption activity.


At 4 weeks, ingrowth of cells from the surrounding tissue into the β-TCP and PLGA/β-TCP biomaterials were observed in the defect area, and new bone formation had started. At 6 weeks, the value for defect closure in the β-TCP group was significantly greater than that in the unfilled control (P < 0.01). A significantly greater level of new bone formation was found in the β-TCP group (P < 0.01) and PLGA/β-TCP group (P < 0.05) than that in the control group, while no significant difference was found between the β-TCP and PLGA/β-TCP groups. At both time points, the height of new tissue/biomaterial in the central third of the defect was significantly increased when the β-TCP or PLGA/β-TCP was used. Proliferating cell nuclear antigen -positive cells were observed around and inside the β-TCP or PLGA/β-TCP, and TRAP-positive cells were found at the surface of the biomaterials, suggesting that remodeling was occurring.


The application of PLGA-coated β-TCP could promote bone regeneration to similar extent as the β-TCP biomaterial in this in vivo model.


bone graft; bone regeneration; poly (lactide-co-glycolide); scaffold; tissue engineering; β-tricalcium phosphate

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