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Virology. 2016 Feb;489:128-40. doi: 10.1016/j.virol.2015.12.003. Epub 2015 Dec 31.

Development of a sheep challenge model for Rift Valley fever.

Author information

1
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, Unites States.
2
United States Department of Agriculture, Agricultural Research Service, Arthropod Borne Animal Disease Research Unit, Manhattan, Kansas, United States.
3
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, Unites States. Electronic address: jricht@vet.k-state.edu.

Abstract

Rift Valley fever (RVF) is a zoonotic disease that causes severe epizootics in ruminants, characterized by mass abortion and high mortality rates in younger animals. The development of a reliable challenge model is an important prerequisite for evaluation of existing and novel vaccines. A study aimed at comparing the pathogenesis of RVF virus infection in US sheep using two genetically different wild type strains of the virus (SA01-1322 and Kenya-128B-15) was performed. A group of sheep was inoculated with both strains and all infected sheep manifested early-onset viremia accompanied by a transient increase in temperatures. The Kenya-128B-15 strain manifested higher virulence compared to SA01-1322 by inducing more severe liver damage, and longer and higher viremia. Genome sequence analysis revealed sequence variations between the two isolates, which potentially could account for the observed phenotypic differences. We conclude that Kenya-128B-15 sheep infection represents a good and virulent challenge model for RVF.

KEYWORDS:

Challenge model; Rift Valley fever; Rift Valley fever virus; Sheep; Strains; Viremia

PMID:
26748334
DOI:
10.1016/j.virol.2015.12.003
[Indexed for MEDLINE]
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