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Int J Pediatr Otorhinolaryngol. 2016 Jan;80:49-52. doi: 10.1016/j.ijporl.2015.11.020. Epub 2015 Nov 24.

Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus.

Author information

1
Department of Otolaryngology-Head & Neck Surgery Georgetown University Hospital, Washington, DC 20007, United States; Georgetown University School of Medicine, Washington, DC 20007, United States. Electronic address: Adw27@georgetown.edu.
2
Department of Otolaryngology-Head & Neck Surgery Georgetown University Hospital, Washington, DC 20007, United States; Georgetown University School of Medicine, Washington, DC 20007, United States.
3
Department of Otolaryngology-Head & Neck Surgery Georgetown University Hospital, Washington, DC 20007, United States; National Institute of Aging, National Institutes of Health, Bethesda, MD 20892, United States.

Abstract

OBJECTIVE:

To determine if Pediatric Autoimmune Neuropsychiatric Disorder Associated with Streptococcus (PANDAS) patients demonstrate a significantly different number of B-Cells or markers of activity when compared to recurrent Group A Streptococcus or Obstructive Sleep Apnea patients.

STUDY DESIGN:

Prospective Cohort Study.

STUDY SETTING:

Academic University Hospital.

METHODS:

Tonsil tissue was collected from twenty-two patients in the operating room and organized into three groups. Ten clinically diagnosed PANDAS, six Group A Streptococcus and six Obstructive Sleep Apnea patients were included in this study. Each tissue sample was extracted with MSD Tris Lysis Buffer and protein lysates were analyzed for CD 19, B-Cell Activating Factor and B-Cell Activating Receptor by western blot methods.

RESULTS:

Based on ANOVA analysis, there was no significant difference in the expression of B-Cell Activating Factor, B-Cell Activating Receptor or CD 19 when comparing the three study groups by western blot analysis methods.

CONCLUSIONS:

In this prospective cohort study, it appears that PANDAS patients do not demonstrate increased number of B-Cells, expression of B-Cell Activating Factor or B-Cell Activating Receptor when compared to Group A Streptococcus or Obstructive Sleep Apnea cohorts. As a result, further evaluation of the cell-mediated immune system is warranted to provide further insight into the pathophysiology of PANDAS. In addition, we must investigate if PANDAS patients only demonstrate increased B-Cell number or activity when undergoing an acute Tic/OCD exacerbation.

KEYWORDS:

Head and neck surgery; Otolaryngology; PANDAS; Pediatrics; Tonsillectomy

PMID:
26746612
DOI:
10.1016/j.ijporl.2015.11.020
[Indexed for MEDLINE]

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