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Biophys J. 2016 Jan 5;110(1):3-6. doi: 10.1016/j.bpj.2015.11.022.

Classic Analysis of Biopolymer Dynamics Is Model Free.

Author information

1
Department of Integrative Structural and Computational Biology MB202, The Scripps Research Institute, La Jolla, California. Electronic address: fenwick@scripps.edu.
2
Department of Integrative Structural and Computational Biology MB202, The Scripps Research Institute, La Jolla, California. Electronic address: dyson@scripps.edu.

Abstract

Early analysis of biopolymer dynamics relied on a variety of motional models that were difficult to distinguish and sometimes gave contradictory results. The Lipari-Szabo model-free approach (documented in a 1980 article in Biophysical Journal, as well as in two more comprehensive 1982 articles in the Journal of the American Chemical Society, provided a simple formalism that allowed investigators to understand fluorescence and NMR experimental data without having to specify a motional model. Although the model-free method is not universally applicable (for example, its assumption of a uniform correlation time for overall molecular tumbling can be problematic for biomolecules containing areas of disorder), it remains the most popular and widely used technique for analyzing molecular motion.

PMID:
26745403
PMCID:
PMC4805862
DOI:
10.1016/j.bpj.2015.11.022
[Indexed for MEDLINE]
Free PMC Article

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