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Ann Biomed Eng. 2016 Jun;44(6):1863-80. doi: 10.1007/s10439-015-1547-5. Epub 2016 Jan 7.

Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage.

Author information

1
Department of Surgery, University of Kansas Medical Center, Kansas City, MO, 66160, USA.
2
Department of Molecular Biosciences, University of Kansas, Lawrence, KS, 66045, USA.
3
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, 77005, USA.
4
Bioengineering Program, University of Kansas, Lawrence, KS, 66045, USA.
5
Department of Chemical and Petroleum Engineering, University of Kansas, 4163 Learned Hall, 1530 W. 15th Street, Lawrence, KS, 66045, USA.
6
Department of Mechanical Engineering, University of Kansas, Lawrence, KS, 66045, USA.
7
Bioengineering Program, University of Kansas, Lawrence, KS, 66045, USA. detamore@ku.edu.
8
Department of Chemical and Petroleum Engineering, University of Kansas, 4163 Learned Hall, 1530 W. 15th Street, Lawrence, KS, 66045, USA. detamore@ku.edu.

Abstract

Hydrogel precursors are liquid solutions that are prone to leaking from the defect site once implanted in vivo. Therefore, the objective of the current study was to create a hydrogel precursor that exhibited a yield stress. Additionally, devitalized cartilage extracellular matrix (DVC) was mixed with DVC that had been solubilized and methacrylated (MeSDVC) to create hydrogels that were chondroinductive. Precursors composed of 10% MeSDVC or 10% MeSDVC with 10% DVC were first evaluated rheologically, where non-Newtonian behavior was observed in all hydrogel precursors. Rat bone marrow stem cells (rBMSCs) were mixed in the precursor solutions, and the solutions were then crosslinked and cultured in vitro for 6 weeks with and without exposure to human transforming growth factor β3 (TGF-β3). The compressive modulus, gene expression, biochemical content, swelling, and histology of the gels were analyzed. The DVC-containing gels consistently outperformed the MeSDVC-only group in chondrogenic gene expression, especially at 6 weeks, where the relative collagen II expression of the DVC-containing groups with and without TGF-β3 exposure was 40- and 78-fold higher, respectively, than that of MeSDVC alone. Future work will test for chondrogenesis in vivo and overall, these two cartilage-derived components are promising materials for cartilage tissue engineering applications.

KEYWORDS:

Chondroinduction; Devitalized cartilage; Hydrogel; Yield stress

PMID:
26744243
PMCID:
PMC5209355
DOI:
10.1007/s10439-015-1547-5
[Indexed for MEDLINE]
Free PMC Article

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