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Sci Rep. 2016 Jan 8;6:19076. doi: 10.1038/srep19076.

Associations of gut-flora-dependent metabolite trimethylamine-N-oxide, betaine and choline with non-alcoholic fatty liver disease in adults.

Author information

1
School of Public Health, Sun Yat-sen University, Guangzhou (510080), People's Republic of China.
2
Faculty of Public Health, School of Medicine, Jinan University, Guangzhou (510632), People's Republic of China.
3
Research and Therapy Center for Liver Disease, the Affiliated Dongnan Hospital of Xiamen University, Zhangzhou (363000), People's Republic of China.
4
Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, University of Sun Yat-Sen, Guangzhou (510120), People's Republic of China.

Abstract

Many studies suggest that trimethylamine-N-oxide (TMAO), a gut-flora-dependent metabolite of choline, contributes to the risk of cardiovascular diseases, but little is known for non-alcoholic fatty liver disease (NAFLD). We examined the association of circulating TMAO, choline and betaine with the presence and severity of NAFLD in Chinese adults. We performed a hospital-based case-control study (CCS) and a cross-sectional study (CSS). In the CCS, we recruited 60 biopsy-proven NAFLD cases and 35 controls (18-60 years) and determined serum concentrations of TMAO, choline and betaine by HPLC-MS/MS. For the CSS, 1,628 community-based adults (40-75 years) completed the blood tests and ultrasonographic NAFLD evaluation. In the CCS, analyses of covariance showed adverse associations of ln-transformed serum levels of TMAO, choline and betaine/choline ratio with the scores of steatosis and total NAFLD activity (NAS) (all P-trend <0.05). The CSS revealed that a greater severity of NAFLD was independently correlated with higher TMAO but lower betaine and betaine/choline ratio (all P-trend <0.05). No significant choline-NAFLD association was observed. Our findings showed adverse associations between the circulating TMAO level and the presence and severity of NAFLD in hospital- and community-based Chinese adults, and a favorable betaine-NAFLD relationship in the community-based participants.

PMID:
26743949
PMCID:
PMC4705470
DOI:
10.1038/srep19076
[Indexed for MEDLINE]
Free PMC Article

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