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J Clin Microbiol. 2016 Mar;54(3):805-8. doi: 10.1128/JCM.03032-15. Epub 2016 Jan 6.

Performance and Verification of a Real-Time PCR Assay Targeting the gyrA Gene for Prediction of Ciprofloxacin Resistance in Neisseria gonorrhoeae.

Author information

1
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
2
Neisseria Reference Laboratory, GISP Regional Laboratory, University of Washington Harborview Medical Center, Seattle, Washington, USA.
3
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA rhumphries@mednet.ucla.edu.
4
Department of Medicine, Division of Infectious Diseases and Department of Epidemiology, David Geffen School of Medicine and Fielding School of Public Health, University of California, Los Angeles, California, USA.

Abstract

In the United States, 19.2% of Neisseria gonorrhoeae isolates are resistant to ciprofloxacin. We evaluated a real-time PCR assay to predict ciprofloxacin susceptibility using residual DNA from the Roche Cobas 4800 CT/NG assay. The results of the assay were 100% concordant with agar dilution susceptibility test results for 100 clinical isolates. Among 76 clinical urine and swab specimens positive for N. gonorrhoeae by the Cobas assay, 71% could be genotyped. The test took 1.5 h to perform, allowing the physician to receive results in time to make informed clinical decisions.

PMID:
26739156
PMCID:
PMC4767994
DOI:
10.1128/JCM.03032-15
[Indexed for MEDLINE]
Free PMC Article

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