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Tumour Biol. 2016 Jul;37(7):8721-9. doi: 10.1007/s13277-015-4737-8. Epub 2016 Jan 7.

Neferine induces autophagy of human ovarian cancer cells via p38 MAPK/ JNK activation.

Author information

1
Department of Cell Biology, Shandong University School of Medicine, 44 Wenhua Xi Road, Jinan, Shandong, 250012, China.
2
Ministry of Education Key Laboratory of Experimental Teratology and Department of Molecular Medicine and Genetics, Shandong University, Jinan, Shandong, China.
3
Hospital Information Center, People's Hospital of Rizhao, Rizhao, Shandong, China.
4
Department of Pathology, Northwestern University School of Medicine, Chicago, IL, USA.
5
Department of Cell Biology, Shandong University School of Medicine, 44 Wenhua Xi Road, Jinan, Shandong, 250012, China. liujian9782@sdu.edu.cn.
6
Ministry of Education Key Laboratory of Experimental Teratology and Department of Molecular Medicine and Genetics, Shandong University, Jinan, Shandong, China. liujian9782@sdu.edu.cn.

Abstract

Ovarian cancer is the most lethal gynecological malignancy. Patients usually have poor prognosis because of late diagnosis, relapse, and chemoresistance. It is pressing to seek novel agent for the treatment of ovarian cancer. Neferine is a bisbenzylisoquinoline alkaloid isolated from the embryos of lotus (Nelumbo nucifera). In this study, we investigated the antitumor effect of neferine on ovarian cancer cells. We found that neferine exhibited growth-inhibitory effect on human ovarian cancer cells, whereas showing less cytotoxic to non-malignant fallopian tube epithelial cells. Furthermore, we demonstrated that neferine induced autophagy and inactivated the mTOR pathway. Finally, we found that both p38 MAPK and JNK signaling pathways were activated by neferine treatment and contributed to the induction of autophagy in ovarian cancer cells. In conclusion, our findings showed that neferine induced autophagy of human ovarian cancer cells via p38 MAPK/JNK activation. Neferine may be explored as a promising antitumoral agent in ovarian cancer.

KEYWORDS:

Autophagy; JNK; Neferine; Ovarian cancer; p38 MAPK

PMID:
26738868
DOI:
10.1007/s13277-015-4737-8
[Indexed for MEDLINE]

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