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Epidemiol Infect. 1989 Aug;103(1):105-12.

Detection of Legionella pneumophila in environmental water samples using a fluorescein conjugated monoclonal antibody.

Author information

1
Department of Medical Microbiology, University of Liverpool.

Abstract

Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. GS detected L. pneumophila in 94% and 100% of environmental water samples which were culture and IFAT positive for L. pneumophila, respectively. IFAT detected 69% of L. pneumophila culture positive samples. Cultures of L. pneumophila serogroups 1 to 12, 14 and non-L. pneumophila bacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. GS identified all serogroups of L. pneumophila and did not cross react with any non-L. pneumophila bacteria. L. pneumophila in environmental samples was easy to detect against a clear dark background when stained with GS.

PMID:
2673821
PMCID:
PMC2249478
DOI:
10.1017/s0950268800030405
[Indexed for MEDLINE]
Free PMC Article

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