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Nat Commun. 2016 Jan 5;7:10184. doi: 10.1038/ncomms10184.

Mouse strains to study cold-inducible beige progenitors and beige adipocyte formation and function.

Berry DC1,2, Jiang Y1,2, Graff JM1,2,3.

Author information

1
Division of Endocrinology, Department of Internal Medicine, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9148, USA.
2
Department of Developmental Biology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, NB5.118, Dallas, Texas 75390-9148, USA.
3
Department of Molecular Biology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9148, USA.

Abstract

Cold temperatures induce formation of beige adipocytes, which convert glucose and fatty acids to heat, and may increase energy expenditure, reduce adiposity and lower blood glucose. This therapeutic potential is unrealized, hindered by a dearth of genetic tools to fate map, track and manipulate beige progenitors and 'beiging'. Here we examined 12 Cre/inducible Cre mouse strains that mark adipocyte, muscle and mural lineages, three proposed beige origins. Among these mouse strains, only those that marked perivascular mural cells tracked the cold-induced beige lineage. Two SMA-based strains, SMA-Cre(ERT2) and SMA-rtTA, fate mapped into the majority of cold-induced beige adipocytes and SMA-marked progenitors appeared essential for beiging. Disruption of the potential of the SMA-tracked progenitors to form beige adipocytes was accompanied by an inability to maintain body temperature and by hyperglycaemia. Thus, SMA-engineered mice may be useful to track and manipulate beige progenitors, beige adipocyte formation and function.

PMID:
26729601
PMCID:
PMC4728429
DOI:
10.1038/ncomms10184
[Indexed for MEDLINE]
Free PMC Article

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