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Nat Rev Genet. 2016 Feb;17(2):81-92. doi: 10.1038/nrg.2015.28. Epub 2016 Jan 5.

Harnessing the power of RADseq for ecological and evolutionary genomics.

Author information

1
Department of Fish and Wildlife Sciences, University of Idaho, 875 Perimeter Drive MS 1136, Moscow, Idaho 83844-1136, USA.
2
University of Montana, Division of Biological Sciences, 32 Campus Dr. HS104, Missoula, Montana 59812, USA.
3
Department of Animal Science, University of California, One Shields Avenue, Davis, California 95616, USA.
4
Flathead Lake Biological Station, Fish and Wildlife Genomics Group, Division of Biological Sciences, University of Montana, Polson, Montana 59860, USA.
5
Institute for Bioinformatics and Evolutionary Studies, Department of Biological Sciences, University of Idaho, Moscow, Idaho 83843, USA.

Abstract

High-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are enabling the low-cost discovery and genotyping of thousands of genetic markers for any species, including non-model organisms, which is revolutionizing ecological, evolutionary and conservation genetics. Technical differences among these methods lead to important considerations for all steps of genomics studies, from the specific scientific questions that can be addressed, and the costs of library preparation and sequencing, to the types of bias and error inherent in the resulting data. In this Review, we provide a comprehensive discussion of RADseq methods to aid researchers in choosing among the many different approaches and avoiding erroneous scientific conclusions from RADseq data, a problem that has plagued other genetic marker types in the past.

PMID:
26729255
PMCID:
PMC4823021
DOI:
10.1038/nrg.2015.28
[Indexed for MEDLINE]
Free PMC Article

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