Format

Send to

Choose Destination
Int J Mol Sci. 2015 Dec 31;17(1). pii: E51. doi: 10.3390/ijms17010051.

Molecular Evolution of Aralkylamine N-Acetyltransferase in Fish: A Genomic Survey.

Li J1,2, You X3, Bian C4,5, Yu H6,7, Coon SL8, Shi Q9,10,11.

Author information

1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. lijia1@genomics.cn.
2
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI, Shenzhen 518083, China. lijia1@genomics.cn.
3
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI, Shenzhen 518083, China. youxinxin@genomics.cn.
4
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI, Shenzhen 518083, China. bianchao@genomics.cn.
5
BGI-Zhenjiang Institute of Hydrobiology, Zhenjiang 212000, China. bianchao@genomics.cn.
6
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. yuhui@genomics.cn.
7
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI, Shenzhen 518083, China. yuhui@genomics.cn.
8
Molecular Genomics Laboratory, National Institutes of Health, Bethesda, MD 20892, USA. coons@mail.nih.gov.
9
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. shiqiong@genomics.cn.
10
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI, Shenzhen 518083, China. shiqiong@genomics.cn.
11
BGI-Zhenjiang Institute of Hydrobiology, Zhenjiang 212000, China. shiqiong@genomics.cn.

Abstract

All living organisms synchronize biological functions with environmental changes; melatonin plays a vital role in regulating daily and seasonal variations. Due to rhythmic activity of the timezyme aralkylamine N-acetyltransferase (AANAT), the blood level of melatonin increases at night and decreases during daytime. Whereas other vertebrates have a single form of AANAT, bony fishes possess various isoforms of aanat genes, though the reasons are still unclear. Here, we have taken advantage of multiple unpublished teleost aanat sequences to explore and expand our understanding of the molecular evolution of aanat in fish. Our results confirm that two rounds of whole-genome duplication (WGD) led to the existence of three fish isoforms of aanat, i.e., aanat1a, aanat1b, and aanat2; in addition, gene loss led to the absence of some forms from certain special fish species. Furthermore, we suggest the different roles of two aanat1s in amphibious mudskippers, and speculate that the loss of aanat1a, may be related to terrestrial vision change. Several important sites of AANAT proteins and regulatory elements of aanat genes were analyzed for structural comparison and functional forecasting, respectively, which provides insights into the molecular evolution of the differences between AANAT1 and AANAT2.

KEYWORDS:

Whole-Genome Duplication (WGD); aralkylamine N-acetyltransferase; gene loss; molecular evolution; phylogenetic analysis; synteny

PMID:
26729109
PMCID:
PMC4730296
DOI:
10.3390/ijms17010051
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center