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J Vet Sci. 2016 Sep 30;17(3):413-9. doi: 10.4142/jvs.2016.17.3.413.

Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model.

Author information

1
College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
2
College of Pharmacy, Kangwon National University, Chuncheon 24341, Korea.
3
Animal Reproduction & Biotechnology Center, Myung-Poom Hanwoo Consulting, Hoengseong 25218, Korea.
4
Department of Biotechnology, College of Engineering, Daegu University, Gyeongsan 38453, Korea.
5
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Korea.

Abstract

Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although the uterus is self-protective, it becomes fragile during periods or pregnancy. To investigate PID, bacteria or lipopolysaccharide (LPS) extracted from gram negative bacteria has been used to induce the disease in several animal models. However, when LPS is applied to the peritoneum, it often causes systemic sepsis leading to death and the PID was not consistently demonstrated. Hydrochloric acid (HCl) has been used to induce inflammation in the lungs and stomach but not tested for reproductive organs. In this study, we developed a PID model in mice by HCl and LPS sequential intracervical (i.c.) administration. The proinflammatory cytokines, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α, were detected in the mouse uterus by western blot analysis and cytokine enzyme-linked immunosorbent assay after HCl (25 mg/kg) administration i.c. followed by four LPS (50 mg/kg) treatments. Moreover, mice exhibited increased infiltration of neutrophils in the endometrium and epithelial layer. These results suggest that ic co-administration of HCl and LPS induces PID in mice. This new model may provide a consistent and reproducible PID model for future research.

KEYWORDS:

hydrochloric acid; lipopolysaccharide; mice; pelvic inflammatory disease; pelvic inflammatory disease model

PMID:
26726020
PMCID:
PMC5037311
DOI:
10.4142/jvs.2016.17.3.413
[Indexed for MEDLINE]
Free PMC Article

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