Format

Send to

Choose Destination
Microb Pathog. 2016 Mar;92:30-35. doi: 10.1016/j.micpath.2015.12.008. Epub 2015 Dec 25.

Gentamicin induces efaA expression and biofilm formation in Enterococcus faecalis.

Author information

1
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Bacteriology, Faculty of Medical Sciences, Tehran, Iran.
2
Department of Bacteriology, Faculty of Medical Sciences, Tehran, Iran. Electronic address: mmmobarez@modares.ac.ir.
3
Department of Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
4
Department of Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
5
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Enterococci have been ranked among the leading causes of nosocomial bacteremia and urinary tract infection. This study aimed to investigate the effect of ampicillin, vancomycin, gentamicin and ceftizoxime on biofilm formation and gene expression of colonization factors on Enterococcus faecalis. Twelve clinical isolates of E. faecalis were used to investigate the effect of antibiotics on biofilm formation and gene expression of efaA, asa1, ebpA, esp and ace. Flow system assay and Microtiter plates were used for biofilm assay. Two hundred clinical isolates were used for confirming the effect of antibiotics on biofilm formation. Ampicillin, vancomycin and ceftizoxime did not have any significant effect on biofilm formation, but gentamicin induced biofilm formation in 89% of isolates. In twelve selected isolate gentamicin increased expression of esp (+50.9%) and efaA (+33.9%) genes and reduced or maintained expression of others (asa1:-47.4%, ebpA: 0, ace:-19.2%). Vancomycin increased expression of esp (+89.1%) but reduced the others (asa1: -34.9%, ebpA:-11%, ace:-30%, efaA:-60%). Ceftizoxime increased slightly ebpA (+19.7%) and reduced others (asa1:-66.2%, esp:-35%, ace:-28.1%, efaA:-38.4%). and ampicillin strongly increased expression of ace (+231%), esp (+131%) and ebpA (+83%) but reduced others (asa1:-85.5%, efaA:-47.4%). The findings of the present study showed that antibiotics may have a role in biofilm formation and sustainability of enterococci, especially in case of gentamicin. efaA gene may have an important role, especially in antibiotic induced biofilm formation by gentamicin. Experiments with efaA mutants are needed to investigate the exact effect of efaA on biofilm formation with antibiotic induced cells.

KEYWORDS:

Antibiotic; Biofilm; Enterococcus faecalis; Expression; Gentamicin

PMID:
26724739
DOI:
10.1016/j.micpath.2015.12.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center