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Int J Clin Exp Pathol. 2015 Oct 1;8(10):12292-9. eCollection 2015.

MicroRNA-485-5p suppresses cell proliferation and invasion in hepatocellular carcinoma by targeting stanniocalcin 2.

Author information

1
Department of General Surgery, Huaihe Hospital of Henan University Kaifeng, Henan Province, China.

Abstract

Increasing evidences indicate that dys-regulation of MicroRNAs contributes to hepatocellular carcinoma. However, the roles of miR-485-5p in HCC are still largely unexplored. In the present study, our quantitative real-time PCR analysis found that miR-485-5p was significantly down-regulated in 50 pairs of human HCC tissues. Moreover, the reduced expression of miR-485-5p was significantly correlated with larger tumor size and more tumor number in patients with HCC. In vitro studies further showed that overexpression of miR-485-5p mimics could inhibit, while its antisense oligos promote cell proliferation and invasion. Results from the dual-luciferase reporter gene assays and western blot further showed that stanniocalcin 2 was a direct target of miR-485-5p. Therefore, our data suggest a novel role for miR-485-5p in the regulation of HCC progression.

KEYWORDS:

Hepatocellular carcinoma; MicroRNA; miR-485-5p; stanniocalcin 2

PMID:
26722415
PMCID:
PMC4680360
[Indexed for MEDLINE]
Free PMC Article

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