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Bioresour Technol. 2016 Mar;203:42-9. doi: 10.1016/j.biortech.2015.12.026. Epub 2015 Dec 17.

Successive pretreatment and enzymatic saccharification of sugarcane bagasse in a packed bed flow-through column reactor aiming to support biorefineries.

Author information

1
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP 12602-810, Lorena, São Paulo, Brazil. Electronic address: teran-2017@usp.br.
2
Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional, CP 07738 Distrito Federal, Mexico.
3
Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional, CP 07738 Distrito Federal, Mexico.
4
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP 12602-810, Lorena, São Paulo, Brazil.

Abstract

A packed bed flow-through column reactor (PBFTCR) was used for pretreatment and subsequent enzymatic hydrolysis of sugarcane bagasse (SCB). Alkaline pretreatment was performed at 70 °C for 4h with fresh 0.3M NaOH solution or with liquor recycled from a previous pretreatment batch. Scheffersomyces stipitis NRRL-Y7124 was used for fermentation of sugars released after enzymatic hydrolysis (20 FPU g(-1) of dry SCB). The highest results for lignin removal were 61% and 52%, respectively, observed when using fresh NaOH or the first reuse of the liquor. About 50% of cellulosic and 57% of hemicellulosic fractions of pretreated SCBs were enzymatically hydrolyzed and the maximum ethanol production was 23.4 g L(-1) (ethanol yield of 0.4 gp gs(-1)), with near complete consumption of both pentoses and hexoses present in the hydrolysate during the fermentation. PBFTCR as a new alternative for SCB-biorefineries is presented, mainly considering its simple configuration and efficiency for operating with a high solid:liquid ratio.

KEYWORDS:

Bioethanol production; Enzymatic saccharification; Packed bed reactor; Reuse of alkaline liquor; Sugarcane bagasse

PMID:
26720138
DOI:
10.1016/j.biortech.2015.12.026
[Indexed for MEDLINE]

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