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BMC Mol Biol. 2015 Dec 29;16:24. doi: 10.1186/s12867-015-0052-6.

microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4.

Li J1,2, Hu L3, Tian C4, Lu F5, Wu J6, Liu L7.

Author information

1
Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Linjiang Road 76, Chongqing, 400010, People's Republic of China. junlichongqing@163.com.
2
Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. junlichongqing@163.com.
3
Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Linjiang Road 76, Chongqing, 400010, People's Republic of China. linahu3321@sina.com.
4
Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. tianchao163@hotmail.com.
5
Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. fenglu1587@sina.com.
6
Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. drjiawu@tom.com.
7
Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China. lililiu122@sina.cn.

Abstract

BACKGROUND:

Dysregulation of microRNA-150 (miR-150) is commonly observed in solid tumor and has been reported to be involved in multiple important biological processes, such as cell proliferation, apoptosis, and metastasis. Elevated miR-150 level was also detected in cervical carcinoma, whereas its function in cancer progression has not been studied yet.

METHODS:

The expression of miRNA-150 in cervical carcinoma was compared with normal cervical tissue and using qRT-PCR. The effects of miR-150 on cell cycle and apoptosis, as well as the expression of cycle- and apoptosis-related genes, were determined using flow cytometry, TUNEL assay, qRT-PCR, and Western blot, respectively. The direct target of miR-150 was confirmed using 3' untranslated region (UTR) luciferase reporter assay.

RESULTS:

miR-150 promotes cervical cancer cell survival and growth, while the inhibition of miR-150 suppresses these actions. miR-150 also induced the cell cycle progression from G1/G0 to S phase, resulting in an enhancement of growth. Several cell cycle- and apoptosis-related genes, CyclinD1, p27, BIM, and FASL were modulated by miR-150. Moreover, miR-150 directly reduced the expression of FOXO4, which regulates the expression of CyclinD1, p27, BIM, and FASL, by targeting its 3' UTR.

CONCLUSION:

Taken together, our data demonstrated that elevated miR-150 targets FOXO4 expression and therefore regulates multiple genes expression, resulting in cervical cancer cell growth and survival.

PMID:
26715362
PMCID:
PMC4696189
DOI:
10.1186/s12867-015-0052-6
[Indexed for MEDLINE]
Free PMC Article

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