Field Trial and Molecular Characterization of RNAi-Transgenic Tomato Plants That Exhibit Resistance to Tomato Yellow Leaf Curl Geminivirus

Mol Plant Microbe Interact. 2016 Mar;29(3):197-209. doi: 10.1094/MPMI-08-15-0181-R. Epub 2016 Feb 11.

Abstract

RNA interference (RNAi) is a widely used approach to generate virus-resistant transgenic crops. However, issues of agricultural importance like the long-term durability of RNAi-mediated resistance under field conditions and the potential side effects provoked in the plant by the stable RNAi expression remain poorly investigated. Here, we performed field trials and molecular characterization studies of two homozygous transgenic tomato lines, with different selection markers, expressing an intron-hairpin RNA cognate to the Tomato yellow leaf curl virus (TYLCV) C1 gene. The tested F6 and F4 progenies of the respective kanamycin- and basta-resistant plants exhibited unchanged field resistance to TYLCV and stably expressed the transgene-derived short interfering RNA (siRNAs) to represent 6 to 8% of the total plant small RNAs. This value outnumbered the average percentage of viral siRNAs in the nontransformed plants exposed to TYLCV-infested whiteflies. As a result of the RNAi transgene expression, a common set of up- and downregulated genes was revealed in the transcriptome profile of the plants selected from either of the two transgenic events. A previously unidentified geminivirus causing no symptoms of viral disease was detected in some of the transgenic plants. The novel virus acquired V1 and V2 genes from TYLCV and C1, C2, C3, and C4 genes from a distantly related geminivirus and, thereby, it could evade the repressive sequence-specific action of transgene-derived siRNAs. Our findings shed light on the mechanisms of siRNA-directed antiviral silencing in transgenic plants and highlight the applicability limitations of this technology as it may alter the transcriptional pattern of nontarget genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Geminiviridae / physiology*
  • Gene Expression Regulation, Plant
  • Genetic Predisposition to Disease
  • Molecular Sequence Data
  • Plant Diseases / genetics
  • Plant Diseases / virology*
  • Plants, Genetically Modified*
  • RNA Interference*
  • RNA, Small Interfering
  • Solanum lycopersicum / genetics*
  • Solanum lycopersicum / physiology*
  • Transcriptome

Substances

  • RNA, Small Interfering

Associated data

  • GENBANK/KM926623
  • GENBANK/KM926624
  • GENBANK/KM926625
  • GENBANK/KM926626
  • GEO/GSE63708