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Mediators Inflamm. 2015;2015:457835. doi: 10.1155/2015/457835. Epub 2015 Dec 2.

Expression of MicroRNAs in the Eyes of Lewis Rats with Experimental Autoimmune Anterior Uveitis.

Author information

1
Department of Ophthalmology, Far Eastern Memorial Hospital, New Taipei City 22056, Taiwan ; Department of Ophthalmology, National Taiwan University Hospital, Taipei 10002, Taiwan ; National Taiwan University College of Medicine, Taipei 10002, Taiwan.
2
National Taiwan University College of Medicine, Taipei 10002, Taiwan.

Abstract

PURPOSE:

This study aimed to determine the dynamic changes of NF-κB-related microRNAs (miRNAs) and cytokines over the course of experimental autoimmune anterior uveitis (EAAU) and elucidate the possible immunopathogenesis.

MATERIALS AND METHODS:

Uveitis was induced in Lewis rats using bovine melanin-associated antigen. The inflammatory activity of the anterior chamber was clinically scored, and leukocytes in the aqueous humor were quantified. RNA was extracted from the iris/ciliary bodies and popliteal lymph nodes to reveal the dynamic changes of eight target miRNAs (miR-155-5p, miR-146a-5p, miR-182-5p, miR-183-5p, miR-147b, miR-21-5p, miR-9-3p, and miR-223-3p) and six cytokine mRNAs (IFN-γ, IL-17, IL-12A, IL-1β, IL-6, and IL-10). In situ hybridization of miRNA and enzyme-linked immunosorbent assay quantification of cytokines were performed to confirm the results.

RESULTS:

Disease activity and leukocyte quantification were maximum at day 15 after immunization. The profiling of miRNA revealed downregulation of miR-146a-5p, miR-155-5p, miR-223-3p, and miR-147b and upregulation of miR-182-5p, miR-183-5p, and miR-9-3p. Cytokine analysis revealed IFN-γ, IL-17, IL-12A, IL-1β, and IL-6 overexpression, with IL-10 downregulation.

CONCLUSIONS:

Dynamic changes of miRNAs were observed over the course of EAAU. By initiating NF-κB signaling, the expressions of downstream cytokines and effector cells from the Th17 and Th1 lineages were sequentially activated, contributing to the disease.

PMID:
26713004
PMCID:
PMC4680116
DOI:
10.1155/2015/457835
[Indexed for MEDLINE]
Free PMC Article

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