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Viruses. 2015 Dec 25;8(1). pii: E6. doi: 10.3390/v8010006.

Quercetin as an Antiviral Agent Inhibits Influenza A Virus (IAV) Entry.

Wu W1, Li R2, Li X3, He J4, Jiang S5,6, Liu S7, Yang J8,9.

Author information

1
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. wj910103@126.com.
2
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. levine091@163.com.
3
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. herelxl@163.com.
4
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. jianhe@smu.edu.cn.
5
Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10065, USA. shibojiang@fudan.edu.cn.
6
Key Lab of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China. shibojiang@fudan.edu.cn.
7
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. liusw@smu.edu.cn.
8
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. yj528@smu.edu.cn.
9
Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10065, USA. yj528@smu.edu.cn.

Abstract

Influenza A viruses (IAVs) cause seasonal pandemics and epidemics with high morbidity and mortality, which calls for effective anti-IAV agents. The glycoprotein hemagglutinin of influenza virus plays a crucial role in the initial stage of virus infection, making it a potential target for anti-influenza therapeutics development. Here we found that quercetin inhibited influenza infection with a wide spectrum of strains, including A/Puerto Rico/8/34 (H1N1), A/FM-1/47/1 (H1N1), and A/Aichi/2/68 (H3N2) with half maximal inhibitory concentration (IC50) of 7.756 ± 1.097, 6.225 ± 0.467, and 2.738 ± 1.931 μg/mL, respectively. Mechanism studies identified that quercetin showed interaction with the HA2 subunit. Moreover, quercetin could inhibit the entry of the H5N1 virus using the pseudovirus-based drug screening system. This study indicates that quercetin showing inhibitory activity in the early stage of influenza infection provides a future therapeutic option to develop effective, safe and affordable natural products for the treatment and prophylaxis of IAV infections.

KEYWORDS:

entry inhibitor; hemagglutinin; influenza A virus; quercetin

PMID:
26712783
PMCID:
PMC4728566
DOI:
10.3390/v8010006
[Indexed for MEDLINE]
Free PMC Article

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