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Environ Microbiol Rep. 2016 Apr;8(2):227-34. doi: 10.1111/1758-2229.12372. Epub 2016 Jan 28.

Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14.

Author information

1
Clinical Research Group 'Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics', OE 6710, Hannover Medical School, Hannover, Germany.
2
Bioinformatics and Systems Biology, Justus-Liebig-Universität, Gießen, Germany.
3
Institute for Medical Microbiology and Hospital Epidemiology, OE 5210, Hannover Medical School, Hannover, Germany.
4
Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), German Center for Lung Research, Hannover, Germany.

Abstract

Bacterial populations differentiate at the subspecies level into clonal complexes. Intraclonal genome diversity was studied in 100 isolates of the two dominant Pseudomonas aeruginosa clones C and PA14 collected from the inanimate environment, acute and chronic infections. The core genome was highly conserved among clone members with a median pairwise within-clone single nucleotide sequence diversity of 8 × 10(-6) for clone C and 2 × 10(-5) for clone PA14. The composition of the accessory genome was, on the other hand, as variable within the clone as between unrelated clones. Each strain carried a large cargo of unique genes. The two dominant worldwide distributed P. aeruginosa clones combine an almost invariant core with the flexible gain and loss of genetic elements that spread by horizontal transfer.

PMID:
26711897
PMCID:
PMC4819714
DOI:
10.1111/1758-2229.12372
[Indexed for MEDLINE]
Free PMC Article

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