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Cell Rep. 2015 Dec 29;13(12):2741-55. doi: 10.1016/j.celrep.2015.11.059. Epub 2015 Dec 17.

Set1 and MLL1/2 Target Distinct Sets of Functionally Different Genomic Loci In Vivo.

Author information

1
Howard Hughes Medical Institute.
2
Department of Biology, University of Oregon, Eugene, OR 97403, USA.
3
Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
4
Howard Hughes Medical Institute; Stowers Institute for Medical Research, Kansas City, MO 64110, USA.

Abstract

Histone H3 lysine 4 trimethylation (H3K4me3) is known to correlate with both active and poised genomic loci, yet many questions remain regarding its functional roles in vivo. We identify functional genomic targets of two H3K4 methyltransferases, Set1 and MLL1/2, in both the stem cells and differentiated tissue of the planarian flatworm Schmidtea mediterranea. We show that, despite their common substrate, these enzymes target distinct genomic loci in vivo, which are distinguishable by the pattern each enzyme leaves on the chromatin template, i.e., the breadth of the H3K4me3 peak. Whereas Set1 targets are largely associated with the maintenance of the stem cell population, MLL1/2 targets are specifically enriched for genes involved in ciliogenesis. These data not only confirm that chromatin regulation is fundamental to planarian stem cell function but also provide evidence for post-embryonic functional specificity of H3K4me3 methyltransferases in vivo.

PMID:
26711341
PMCID:
PMC4707048
DOI:
10.1016/j.celrep.2015.11.059
[Indexed for MEDLINE]
Free PMC Article

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