Format

Send to

Choose Destination
Cell Rep. 2015 Dec 29;13(12):2679-86. doi: 10.1016/j.celrep.2015.11.058. Epub 2015 Dec 17.

Directed Migration of Pulmonary Neuroendocrine Cells toward Airway Branches Organizes the Stereotypic Location of Neuroepithelial Bodies.

Author information

1
Laboratory for Lung Development, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
2
Laboratory for Mouse Genetic Engineering, RIKEN Quantitative Biology Center, BioSysytems Building, Osaka university, 1-3 Yamadaoka, Suita, Osaka, 565-0871 Japan.
3
Laboratory for Lung Development, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan. Electronic address: mmorimoto@cdb.riken.jp.

Abstract

The airway epithelium consists of diverse cell types, including neuroendocrine (NE) cells. These cells are thought to function as chemoreceptors and as a component of the stem cell niche as well as the cells of origin in small-cell lung cancer. NE cells often localize at bifurcation points of airway tubes, forming small clusters called neuroepithelial bodies (NEBs). To investigate NEB development, we established methods for 3D mapping and ex vivo 4D imaging of developing lungs. We found that NEBs localize at stereotypic positions in the bifurcation area irrespective of variations in size. Notch-Hes1 signaling contributes to the differentiation of solitary NE cells, regulating their number but not localization. Live imaging revealed that individual NE cells migrate distally to and cluster at bifurcation points, driving NEB formation. We propose that NEB development is a multistep process involving differentiation of individual NE cells and their directional migration to organize NEBs.

PMID:
26711336
DOI:
10.1016/j.celrep.2015.11.058
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center