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Exp Neurol. 2016 Mar;277:96-102. doi: 10.1016/j.expneurol.2015.12.009. Epub 2015 Dec 18.

Effects of serum immunoglobulins from patients with complex regional pain syndrome (CRPS) on depolarisation-induced calcium transients in isolated dorsal root ganglion (DRG) neurons.

Author information

1
Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK.
2
Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK; Pain Research Institute, Department of Translational Medicine, University of Liverpool, Liverpool, L9 7AL, UK.
3
Pain Research Institute, Department of Translational Medicine, University of Liverpool, Liverpool, L9 7AL, UK.
4
Pain Research Institute, Department of Translational Medicine, University of Liverpool, Liverpool, L9 7AL, UK. Electronic address: andreasgoebel@rocketmail.com.

Abstract

Complex regional pain syndrome (CRPS) is thought to have an auto-immune component. One such target recently proposed from the effects of auto-immune IgGs on Ca(2+) transients in cardiac myocytes and cell lines is the α1-adrenoceptor. We have tested whether such IgGs exerted comparable effects on nociceptive sensory neurons isolated from rat dorsal root ganglia. Depolarisation-induced [Ca(2+)]i transients were generated by applying 30 mM KCl for 2 min and monitored by Fura-2 fluorescence imaging. No IgGs tested (including 3 from CRPS patients) had any significant effect on these [Ca(2+)]i transients. However, IgG from one CRPS patient consistently and significantly reduced the K(+)-induced response of cells that had been pre-incubated for 24h with a mixture of inflammatory mediators (1 μM histamine, 5-hydroxytryptamine, bradykinin and PGE2). Since this pre-incubation also appeared to induce a comparable inhibitory response to the α1-agonist phenylephrine, this is compatible with the α1-adrenoceptor as a target for CRPS auto-immunity. A mechanism whereby this might enhance pain is suggested.

KEYWORDS:

Auto-immunity; Calcium; Complex regional pain syndrome; Immunoglobulin; Sensory neurons

PMID:
26708558
DOI:
10.1016/j.expneurol.2015.12.009
[Indexed for MEDLINE]

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