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Exp Cell Res. 2016 Jan 1;340(1):150-8. doi: 10.1016/j.yexcr.2015.12.009. Epub 2015 Dec 18.

Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis.

Author information

1
Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
2
Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Trento, Italy.
3
Department of Computer Science, University of Verona, Verona, Italy.
4
Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy. Electronic address: raffaella.mariotti@univr.it.

Abstract

Therapeutic strategies for the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS) have not yet provided satisfactory results. Interest in stem cells for the treatment of neurodegenerative diseases is increasing and their beneficial action seems to be due to a paracrine effect via the release of exosomes, main mediators of cell-cell communication. Here we wished to assess, in vitro, the efficacy of a novel non-cell therapeutic approach based on the use of exosomes derived from murine adipose-derived stromal cells on motoneuron-like NSC-34 cells expressing ALS mutations, and used as in vitro models of disease. In particular, we set out to investigate the effect of exosomes on NSC-34 naïve cells and NSC-34 cells overexpressing human SOD1(G93A) or SOD1(G37R) or SOD1(A4V) mutants, exposed to oxidative stress. The data presented here indicate for the first time that exosomes (0.2 µg/ml) are able to protect NSC-34 cells from oxidative damage, which is one of the main mechanism of damage in ALS, increasing cell viability. These data highlight a promising role of exosomes derived from stem cells for potential therapeutic applications in motoneuron disease.

KEYWORDS:

ALS; Extracellular vesicles; Mesenchymal stem cell; Motoneuron disease; Motoneuron-like NSC-34; Paracrine effect

PMID:
26708289
DOI:
10.1016/j.yexcr.2015.12.009
[Indexed for MEDLINE]

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