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Infect Genet Evol. 2016 Mar;38:90-95. doi: 10.1016/j.meegid.2015.12.013. Epub 2015 Dec 18.

Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses.

Author information

1
Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920-3101, Japan.
2
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
3
Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
4
Tochigi Prefectural Central District Animal Hygiene Service Center, Hiraidekougyoudanchi, Utsunomiya, Tochigi 321-0905, Japan.
5
Kurayoshi Livestock Hygiene Service Center, Kiyotani, Kurayoshi, Tottori 683-0017, Japan.
6
Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan.
7
Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
8
Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
9
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
10
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. Electronic address: m-nagai@cc.tuat.ac.jp.

Abstract

Bovine toroviruses (BToVs), belong to the subfamily Toroviridae within the family Coronaviridae, and are pathogens, causing enteric disease in cattle. In Japan, BToVs are distributed throughout the country and cause gastrointestinal infection of calves and cows. In the present study, complete genome sequences of two Japanese BToVs and partial genome sequences of two Japanese BToVs and one porcine torovirus (PToV) from distant regions in Japan were determined and genetic analyses were performed. Pairwise nucleotide comparison and phylogenetic analyses revealed that Japanese BToVs shared high identity with each other and showed high similarities with BToV Breda1 strain in S, M, and HE coding regions. Japanese BToVs showed high similarities with porcine toroviruses in ORF1a, ORF1b, and N coding regions and the 5' and 3' untranslated regions, suggestive of a natural recombination event. Recombination analyses mapped the putative recombinant breakpoints to the 3' ends of the ORF1b and HE regions. These findings suggest that the interspecies recombinant nature of Japanese BToVs resulted in a closer relationship between BToV Breda1 and PToVs.

KEYWORDS:

Bovine torovirus; Japan; Natural recombination; Porcine torovirus

PMID:
26708248
DOI:
10.1016/j.meegid.2015.12.013
[Indexed for MEDLINE]

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