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J Biotechnol. 2016 Oct 10;235:132-8. doi: 10.1016/j.jbiotec.2015.12.015. Epub 2015 Dec 18.

Synergism of proteomics and mRNA sequencing for enzyme discovery.

Author information

1
Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria.
2
Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; Medical University of Graz, Institute of Pathology, Research Unit Functional Proteomics and Metabolic Pathways, Stiftingtalstrasse 24, 8010 Graz, Austria; Omics Center Graz, BioTechMed-Graz, Stiftingtalstrasse 24, 8010 Graz, Austria.
3
Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; TU Graz, Institute of Molecular Biotechnology, NAWI Graz, Petersgasse 14, 8010 Graz, Austria.
4
Austrian Center of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; Medical University of Graz, Institute of Pathology, Research Unit Functional Proteomics and Metabolic Pathways, Stiftingtalstrasse 24, 8010 Graz, Austria; Omics Center Graz, BioTechMed-Graz, Stiftingtalstrasse 24, 8010 Graz, Austria. Electronic address: ruth.birner-gruenberger@medunigraz.at.

Abstract

Enzyme catalyzed processes are increasingly complementing chemical manufacturing as new enzymes are being discovered. Although, many industrially applied biocatalysts have been identified by functional screenings technological advances in the omics fields have created a different path to access novelty. Here we describe how omics technologies, especially proteomics and transcriptomics, can complement each other in the aim of finding new enzymatic functions. Special emphasis is laid on how mRNA sequencing Zcan improve proteomic experiments by allowing the generation of high quality protein sequence databases, which subsequently facilitates protein identification.

KEYWORDS:

Biotechnology; Database; Enzyme discovery; Proteomics; Transcriptomics

PMID:
26707808
DOI:
10.1016/j.jbiotec.2015.12.015
[Indexed for MEDLINE]
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