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Int J Biol Macromol. 2016 Mar;84:295-300. doi: 10.1016/j.ijbiomac.2015.12.034. Epub 2015 Dec 17.

Substituted galacturonan from starfruit: Chemical structure and antinociceptive and anti-inflammatory effects.

Author information

1
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil.
2
Departamento de Ciências Fisiológicas, Universidade Federal de Santa Catarina, CEP 88040-900, Florianópolis, SC, Brazil.
3
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil. Electronic address: lucimaramcc@ufpr.br.

Abstract

Starfruit (Averrhoa carambola L.) is an edible tropical fruit, which is usually consumed as a fresh table fruit or as fruit juice. It also exhibits various pharmacological activities. In this study, polysaccharides were extracted with boiling water and purified by freeze-thawing and Fehling treatments. After purification steps, a homogenous fraction was obtained. It was analyzed by sugar composition, gel permeation chromatography, methylation, and two-dimensional nuclear magnetic resonance (2D NMR) spectroscopy analyses. It comprised arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in a molar ratio of 12.3:1.7:86.0. Methylation and NMR spectroscopy analyses showed that it contained a substituted galacturonan composed of (1→4)-linked α-D-Galp A units branched at O-2 by (1→5)-linked α-L-Araf and terminal α-L-Araf and α-D-Galp A units. The effect of PFSCW (10-300mg/kg, i.p.) on nocifensive behavior induced by intraplantar injection of formalin in mice was evaluated. The fraction demonstrated antinociceptive and anti-inflammatory properties, suggesting that it may be useful in therapeutic intervention for the management of inflammatory pain.

KEYWORDS:

Anti-inflammatory and antinociceptive activities; Starfruit; Substituted galacturonans

PMID:
26706843
DOI:
10.1016/j.ijbiomac.2015.12.034
[Indexed for MEDLINE]

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