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Biol Trace Elem Res. 2016 Aug;172(2):354-60. doi: 10.1007/s12011-015-0604-0. Epub 2015 Dec 26.

Expression of Selenoprotein Genes Is Affected by Heat Stress in IPEC-J2 Cells.

Author information

1
Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
2
Sichuan Provincial General Station for Animal Husbandry, Chengdu, 610041, China.
3
Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China. zhua666@126.com.

Abstract

The aim of this study was to explore the impacts of heat stress (HS) on expressions of selenoprotein genes in IPEC-J2 cells. Cells were cultured with 5 % CO2-humidified chamber at 37 °C until the cells grew to complete confluence and then exposed to a mild hyperthermia at 41.5 °C (HS) or 37 °C (control) for another 24 h, finally harvested for total RNA or protein extraction. Real-time quantitative PCRs (qPCRs) were performed to compare gene expression of 25 selenoprotein genes, 3 tight junction-related genes, and 10 inflammation-related genes. Protein expressions of heat shock protein 70 (Hsp70) and selenoprotein X and P (SelX and SelP) were also investigated by Western blot. The results showed that HS up-regulated (P < 0.05) Hsp70 and one tight junction-related gene [zonula occludens-1 (Zo-1)] in IPEC-J2 cells. At the same time, HS up-regulated (P < 0.05) 4 selenoprotein genes (Gpx3, Dio2, Selk, Sels) and three inflammation-related genes (Il-6, Icam-1, Tgf-β) and down-regulated (P < 0.05 or as indicated) six selenoprotein genes (Gpx2, Gpx6, Txnrd1, Selh, Selm, Selx) and three inflammation-related genes (Ifn-β, Mcp-1, Tnf-α) in the cells. HS also exhibited impacts on protein expressions, which up-regulated Hsp70, down-regulated SelX, and showed no effect on SelP in IPEC-J2 cells. Our results showed that HS affected the expression of inflammation-related genes and up-regulated gene and protein expressions of Hsp70. The changes of so many selenoprotein genes expression implied a potential link between selenoprotein genes and HS. Moreover, the results provided by this IPEC-J2 model may be used to further study the interactive mechanisms between selenoprotein function and potential intestinal damage induced by HS.

KEYWORDS:

Expression; Heat stress; IPEC-J2; Selenoprotein

PMID:
26706036
DOI:
10.1007/s12011-015-0604-0
[Indexed for MEDLINE]

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