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J Ethnopharmacol. 2016 Feb 3;178:222-8. doi: 10.1016/j.jep.2015.12.016. Epub 2015 Dec 17.

Polyphenols from Cymbopogon citratus leaves as topical anti-inflammatory agents.

Author information

1
Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Portugal; Center for Neurosciences and Cell Biology, University of Coimbra, Portugal. Electronic address: costagff@gmail.com.
2
Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Portugal; Center for Neurosciences and Cell Biology, University of Coimbra, Portugal.
3
CIEPQPF, Research Center for Chemical Processes Engineering and Forest Products, Chemical Engineering Department, Faculty of Sciences and Technology, University of Coimbra, Portugal.
4
Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Portugal; Institute for Biomedical Imaging and Life Sciences, University of Coimbra, Portugal.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

A variety of plant polyphenols have been reported to have anti-inflammatory, frequently associated with erythema, edema, hyperplasia, skin photoaging and photocarcinogenesis. Cymbopogon citratus (DC). Stapf (Poaceae) is a worldwide known medicinal plant, used in traditional medicine in inflammation-related conditions.

AIM OF THE STUDY:

In this work, the anti-inflammatory potential of C. citratus infusion (CcI) and its polyphenols as topical agents was evaluated in vivo.

MATERIALS AND METHODS:

The plant extract was prepared and its fractioning led two polyphenol-rich fractions: flavonoids fraction (CcF) and tannins fraction (CcT). An oil/water emulsion was developed with each active (CcI, CcF+CcT and diclofenac), pH and texture having been evaluated. Release tests were further performed using static Franz diffusion cells and all collected samples were monitored by HPLC-PDA. In vivo topical anti-inflammatory activity evaluation was performed by the carrageenan-induced rat paw edema model.

RESULTS:

The texture analysis revealed statistically significant differences for all tested parameters to CcF+CcT, supporting its topical application. Release experiments lead to the detection of the phenolic compounds from each sample in the receptor medium and the six major flavonoids were quantified, by HPLC-PDA: carlinoside, isoorientin, cynaroside, luteolin 7-O-neohesperidoside, kurilesin A and cassiaoccidentalin B. The CcF+CcT formulation prompted to the higher release rate for all these flavonoids. CcI4%, CcI1% and CcF+CcT exhibited an edema reduction of 43.18, 29.55 and 59.09%, respectively.

CONCLUSIONS:

Our findings highlight that CcI, containing luteolin 7-O-neohesperidoside, cassiaoccidentalin B, carlinoside, cynaroside and tannins have a potential anti-inflammatory topical activity, suggesting their promising application in the treatment of skin inflammatory pathologies.

KEYWORDS:

Anti-inflammatory; Cymbopogon citratus; Drug release; Lemongrass; Topical application; in vivo studies

PMID:
26702504
DOI:
10.1016/j.jep.2015.12.016
[Indexed for MEDLINE]

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