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Tumour Biol. 2016 Jun;37(6):7915-20. doi: 10.1007/s13277-015-4688-0. Epub 2015 Dec 23.

MicroRNA-148a represents an independent prognostic marker in bladder cancer.

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Department of Urology, Qilu Hospital of Shan Dong University, No. 107 Wenhuaxi Road, Jinan City, 250012, China.
Department of Urology, Reproductive Hospital Affiliated to Shandong University, Jinan, China.
Department of Urology, Qilu Hospital of Shan Dong University, No. 107 Wenhuaxi Road, Jinan City, 250012, China.


A previous study has demonstrated the roles of microRNA-148a (miR-148a) on apoptosis of bladder cancer cells. The goal of this study was to investigate whether the miR-148a expression level could serve as a new biomarker for the prognosis of bladder cancer patients. We collected a total of 126 bladder cancer samples. The expression level of miR-148a was determined with quantitative real-time polymerase chain reaction (qRT-PCR). Kaplan-Meier method was used to analyze the overall survival. Cox regression analysis was further used to identify prognostic factors. The expression levels of miR-148a in bladder cancer tissues were identified (1.5 ± 0.3; P < 0.001). The bladder cancer patients in the low-expression group more frequently had a high tumor grade (P = 0.025), increased tumor recurrence (P = 0.002), and advanced lymph node (LN) metastasis (P = 0.001). Patient survival analysis revealed a clear positive correlation between miR-148a expression level and survival time of bladder cancer patients (P = 0.005, log-rank = 7.714). In univariate Cox proportional hazards regression analysis, we found that a low-expression level of miR-148a (P = 0.018), tumor grade (P = 0.006), lymph node metastasis (P = 0.001), and recurrence (P < 0.001) were associated with the prognosis of bladder cancer. In multivariate analysis, we found that miR-148a expression (RR = 0.206, 95 % CI 0.095-0.813, P = 0.029), tumor grade (RR = 0.714, 95 % CI 0.224-0.958, P = 0.714), lymph node metastasis (RR = 6.604, 95 % CI 3.192-12.547, P < 0.001), and recurrence (RR = 15.126, 95 % CI 6.714-22.025, P < 0.001) retained significance as an independent prognostic factor of bladder cancer survival (Table 3). All results have showed that miR-148a expression was decreased in bladder cancer specimens and reduced miR-148a expression was associated with poorer survival time, indicating that miR-148a may become a candidate factor for predicting the prognosis of bladder cancer.


Bladder cancer; Prognosis; Survival; miR-148a

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