Send to

Choose Destination
J Extracell Vesicles. 2015 Dec 23;4:29509. doi: 10.3402/jev.v4.29509. eCollection 2015.

High-speed centrifugation induces aggregation of extracellular vesicles.

Author information

Molecular Imaging and NanoBioTechnology, University of Bordeaux, Pessac, France.
Institut Universitaire de France, Paris, France;


Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins.


blood plasma; centrifugation; cryo-electron microscopy; extracellular vesicles; flow cytometry; immuno-gold electron microscopy; vesicle aggregation

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center