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Genom Data. 2015 Sep 16;6:199-201. doi: 10.1016/j.gdata.2015.09.015. eCollection 2015 Dec.

Generating and evaluating a ranked candidate gene list for potential vertebrate heart field regulators.

Author information

1
Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
2
Howard Hughes Medical Institute, Boston, MA 02115, USA ; Stem Cell Program, Boston Children's Hospital, MA 02115, USA ; Division of Hematology/Oncology, Boston Children's Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA ; Institute of Molecular Life Sciences (IMLS), University of Zürich, 8057 Zürich, Switzerland.
3
Max-Delbrück Center for Molecular Medicine (MDC), 13125 Berlin, Buch, Germany.
4
Institute of Molecular Life Sciences (IMLS), University of Zürich, 8057 Zürich, Switzerland.
5
Howard Hughes Medical Institute, Boston, MA 02115, USA ; Stem Cell Program, Boston Children's Hospital, MA 02115, USA ; Division of Hematology/Oncology, Boston Children's Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA.

Abstract

The vertebrate heart develops from two distinct lineages of cardiomyocytes that arise from the first and second heart fields (FHF and SHF, respectively). The FHF forms the primitive heart tube, while adding cells from the SHF allows elongation at both poles of the tube. Initially seen as an exclusive characteristic of higher vertebrates, recent work has demonstrated the presence of a distinct FHF and SHF in lower vertebrates, including zebrafish. We found that key transcription factors that regulate septation and chamber formation in higher vertebrates, including Tbx5 and Pitx2, influence relative FHF and SHF contributions to the zebrafish heart tube. To identify molecular modulators of heart field migration, we used microarray-based expression profiling following inhibition of tbx5a and pitx2ab in embryonic zebrafish (Mosimann & Panakova, et al, 2015; GSE70750). Here, we describe in more detail the procedure used to process, prioritize, and analyze the expression data for functional enrichment.

KEYWORDS:

Genomics; Heart development; Zebrafish; pitx2; tbx5

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