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Iran J Microbiol. 2015 Aug;7(4):214-20.

Evolution of hepatitis B virus surface gene and protein among Iranian chronic carriers from different provinces.

Author information

1
Hepatitis B Molecular Laboratory-Department of Virology-School of Public Health-Tehran University of Medical Sciences, Tehran, Iran.
2
Middle East Liver Diseases Center (MELD Centers), Tehran, Iran.
3
Mental health research center, Iran University of Medical Sciences, Tehran, Iran.
4
Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran.
5
Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
6
Hepatitis Research Center, Department of Internal Medicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
7
Department of Infectious Disease, Mashhad University of Medical Sciences, Iran.
8
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
9
Kermanshah Liver Diseases and Hepatitis Research Center, Kermanshah, Iran.
10
Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
11
Educational Region of Khozestan Blood Transfusion Organization, Ahvaz, Iran.
12
Department of Gastroenterology, Zahedan University of Medical Sciences, Zahedan, Iran.
13
Gastrointestinal & Liver Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran.
14
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
15
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
16
Gholhack medical laboratory Tehran, Iran.
17
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
18
Ministry of Health and Medical Education, Deputy of Curative Affairs, Budget Administration, Tehran, Iran.

Abstract

BACKGROUND AND OBJECTIVES:

Iranian chronic HBV carrier's population has shown a unique pattern of genotype D distribution all around the country. The aim of this study was to explore more details of evolutionary history of carriers based on structural surface proteins from different provinces.

MATERIALS AND METHODS:

Sera obtained from 360 isolates from 12 Different regions of country were used for amplification and sequencing of surface proteins. A detailed mutational analysis was undertaken.

RESULTS:

The total ratio for Missense/Silent nucleotide substitutions was 0.96. Sistan and Kermanshah showed the lowest rate of evolution between provinces (P = 0.055). On the other hand, Khorasan Razavi and Khoozestan contained the highest ratio (P = 0.055). The rest of regions were laid between these two extremes. Azarbayjan and Guilan showed the highest proportion of immune epitope distribution (91.3% and 96%, respectively). Conversely, Sistan and Tehran harbored the least percentage (66.6% and 68.8%, respectively). Kermanshah province contained only 5.2%, whereas Isfahan had 54.5% of B cell epitope distribution. In terms of T helper epitopes, all provinces showed a somehow homogeneity: 22.58% (Fars) to 46.6% (Khuzestan). On the other hand, distribution of substitutions within the CTL epitopes showed a wide range of variation between 6.6% (Khuzestan) and 63% (Kermanshah).

CONCLUSION:

Further to low selection pressure found in Iranian population, the variations between different regions designate random genetic drift within the surface proteins. These finding would have some applications in terms of specific antiviral regimen, design of more efficient vaccine and public health issues.

KEYWORDS:

HBsAg; Hepatitis B; Surface Proteins

PMID:
26697161
PMCID:
PMC4685166

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