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J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.

The nuclear basket mediates perinuclear mRNA scanning in budding yeast.

Author information

1
Departement de Biochimie et Medecine Moleculaire, Faculte de Medecine, Universite de Montreal, H3T 1J4 Montreal, Quebec, Canada.
2
Departement de Biochimie et Medecine Moleculaire, Faculte de Medecine, Universite de Montreal, H3T 1J4 Montreal, Quebec, Canada Institut de Recherches Cliniques de Montreal, H2W 1R7 Montreal, Quebec, Canada.
3
Montreal Neurological Institute, McGill University, H3A 2B4 Montreal, Quebec, Canada.
4
Departement de Biochimie et Medecine Moleculaire, Faculte de Medecine, Universite de Montreal, H3T 1J4 Montreal, Quebec, Canada Institut de Recherches Cliniques de Montreal, H2W 1R7 Montreal, Quebec, Canada Faculty of Medicine, Division of Experimental Medicine, McGill University, H3A 2B4 Montreal, Quebec, Canada.
5
Departement de Biochimie et Medecine Moleculaire, Faculte de Medecine, Universite de Montreal, H3T 1J4 Montreal, Quebec, Canada daniel.r.zenklusen@umontreal.ca.

Abstract

After synthesis and transit through the nucleus, messenger RNAs (mRNAs) are exported to the cytoplasm through the nuclear pore complex (NPC). At the NPC, messenger ribonucleoproteins (mRNPs) first encounter the nuclear basket where mRNP rearrangements are thought to allow access to the transport channel. Here, we use single mRNA resolution live cell microscopy and subdiffraction particle tracking to follow individual mRNAs on their path toward the cytoplasm. We show that when reaching the nuclear periphery, RNAs are not immediately exported but scan along the nuclear periphery, likely to find a nuclear pore allowing export. Deletion or mutation of the nuclear basket proteins MLP1/2 or the mRNA binding protein Nab2 changes the scanning behavior of mRNPs at the nuclear periphery, shortens residency time at nuclear pores, and results in frequent release of mRNAs back into the nucleoplasm. These observations suggest a role for the nuclear basket in providing an interaction platform that keeps RNAs at the periphery, possibly to allow mRNP rearrangements before export.

PMID:
26694838
PMCID:
PMC4687876
DOI:
10.1083/jcb.201503070
[Indexed for MEDLINE]
Free PMC Article

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