Multiprotein Printing by Light-Induced Molecular Adsorption

Adv Mater. 2016 Mar 9;28(10):2024-9. doi: 10.1002/adma.201504154. Epub 2015 Dec 21.

Abstract

Light-induced molecular adsorption of proteins (LIMAP) allows for quantitative sub-micrometer-resolution printing of multiple biomolecules. Surface-bound gradients are patterned within minutes over an entire glass cover-slip. LIMAP is used to perform selective immuno-assays, to dynamically control the adhesion of individual cells, and to achieve hierarchical co-cultures instrumental for tissue engineering.

Keywords: cell co-culture; photopatterning; poly(ethylene glycol); protein micropatterning; single molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Cell Adhesion
  • Cell Line
  • Fibronectins / chemistry
  • Fibronectins / metabolism
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / metabolism
  • Light*
  • Mice
  • Microscopy, Atomic Force
  • Microscopy, Fluorescence
  • Molecular Imprinting
  • Proteins / chemistry*
  • Proteins / metabolism
  • Surface Properties
  • Tissue Engineering

Substances

  • Fibronectins
  • Proteins
  • Green Fluorescent Proteins