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J Agric Food Chem. 2016 Jan 13;64(1):171-7. doi: 10.1021/acs.jafc.5b04878. Epub 2015 Dec 28.

Identification and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in China by UPLC-PDA and UPLC-QTOF-MS/MS.

Author information

1
State Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, China.
2
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University , Wuxi, Jiangsu 214122, China.
3
School of Food and Nutrition Engineering, Jiangsu Food and Pharmaceutical Science College , Huaian, Jiangsu 223003, China.
4
Xuzhou Sweetpotato Research Institute, Chinese Academy of Agricultural Science , Xuzhou, Jiangsu 221131, China.

Abstract

The identification and quantitation of the anthocyanins in 12 purple-fleshed sweet potato (PFSP) cultivars ('Jihei 1', 'Xuzi 3', 'Xuzi 6', 'Zhezi 4', 'Ningzi 1', 'Ningzi 2', 'Ningzi 3', 'Ning 2-2', 'Ning 6-8', 'Guangzi 1', 'Ziluolan', and 'Qinzi 1') in China were carried out using a combination of ultraperformance liquid chromatography-photodiode array (UPLC-PDA), quadrupole-time-of-flight mass spectrometry (QTOF-MS), and tandem mass spectrometry (MS/MS) analyses. Thirteen acylated anthocyanins were tentatively characterized, including two new PFSP anthocyanins, cyanidin 3-caffeoyl-vanilloyl sophoroside-5-glucoside and peonidin 3-caffeoyl-vanilloyl sophoroside-5-glucoside. The quantitative analyses of these anthocyanins were conducted using cyanidin 3-O-glucoside as a standard. The total anthocyanin content of the PFSPs depended on the cultivar. The five PFSP cultivars with the highest content of anthocyanins were 'Jihei 1', 'Xuzi 3', 'Zhezi 4', 'Ziluolan', and 'Qinzi 1'. This is the first report of the 'Ningzi 2', 'Ningzi 3', and 'Ning 2-2' PFSP cultivars containing only diacylated anthocyanins and of the 'Xuzi 6' cultivar containing single anthocyanidin-based anthocyanins.

KEYWORDS:

UPLC-PDA; UPLC-QTOF-MS/MS; anthocyanin; identification; purple-fleshed sweet potato; quantitation

PMID:
26687974
DOI:
10.1021/acs.jafc.5b04878
[Indexed for MEDLINE]

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