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Cell. 2015 Dec 17;163(7):1770-1782. doi: 10.1016/j.cell.2015.11.022.

Control of Synaptic Connectivity by a Network of Drosophila IgSF Cell Surface Proteins.

Author information

1
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.
2
Dept. of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL.
3
Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA.
4
Howard Hughes Medical Institute, Program in Developmental Biology, Dept. of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at TCH, Baylor College of Medicine, Houston, TX.
#
Contributed equally

Abstract

We have defined a network of interacting Drosophila cell surface proteins in which a 21-member IgSF subfamily, the Dprs, binds to a nine-member subfamily, the DIPs. The structural basis of the Dpr-DIP interaction code appears to be dictated by shape complementarity within the Dpr-DIP binding interface. Each of the six dpr and DIP genes examined here is expressed by a unique subset of larval and pupal neurons. In the neuromuscular system, interactions between Dpr11 and DIP-γ affect presynaptic terminal development, trophic factor responses, and neurotransmission. In the visual system, dpr11 is selectively expressed by R7 photoreceptors that use Rh4 opsin (yR7s). Their primary synaptic targets, Dm8 amacrine neurons, express DIP-γ. In dpr11 or DIP-γ mutants, yR7 terminals extend beyond their normal termination zones in layer M6 of the medulla. DIP-γ is also required for Dm8 survival or differentiation. Our findings suggest that Dpr-DIP interactions are important determinants of synaptic connectivity.

KEYWORDS:

GFP tagging; Mimic; cell adhesion; immunoglobulin; optic lobe; synaptogenesis

PMID:
26687361
PMCID:
PMC4720259
DOI:
10.1016/j.cell.2015.11.022
[Indexed for MEDLINE]
Free PMC Article

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