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Cell Rep. 2015 Dec 22;13(11):2336-2344. doi: 10.1016/j.celrep.2015.11.041. Epub 2015 Dec 10.

Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase.

Author information

1
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
2
Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
3
Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK. Electronic address: luis.aragon@csc.mrc.ac.uk.

Abstract

Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin's DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation.

PMID:
26686624
PMCID:
PMC4695335
DOI:
10.1016/j.celrep.2015.11.041
[Indexed for MEDLINE]
Free PMC Article

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