Format

Send to

Choose Destination
J Am Chem Soc. 2016 Jan 13;138(1):402-7. doi: 10.1021/jacs.5b11357. Epub 2016 Jan 4.

Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11.

Author information

1
Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.
2
Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.
3
Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
4
School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore.
5
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University , Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.
6
Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University , 8000 Aarhus C, Denmark.
7
Interdisciplinary Graduate School, Nanyang Technological University , 637551, Singapore.
8
Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen , Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.

Abstract

Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

PMID:
26684612
DOI:
10.1021/jacs.5b11357
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center