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Appl Biochem Biotechnol. 2016 Apr;178(7):1325-38. doi: 10.1007/s12010-015-1949-8. Epub 2015 Dec 18.

Electrokinetic-Enhanced Remediation of Phenanthrene-Contaminated Soil Combined with Sphingomonas sp. GY2B and Biosurfactant.

Lin W1, Guo C2,3, Zhang H1, Liang X1, Wei Y1, Lu G1,4, Dang Z1,5.

Author information

1
School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China.
2
School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China. clguo@scut.edu.cn.
3
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou, 510006, People's Republic of China. clguo@scut.edu.cn.
4
Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, Guangzhou, 510006, People's Republic of China.
5
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou, 510006, People's Republic of China.

Abstract

Electrokinetic-microbial remediation (EMR) has emerged as a promising option for the removal of polycyclic aromatic hydrocarbons (PAHs) from contaminated soils. The aim of this study was to enhance degradation of phenanthrene (Phe)-contaminated soils using EMR combined with biosurfactants. The electrokinetic (EK) remediation, combined with Phe-degrading Sphingomonas sp. GY2B, and biosurfactant obtained by fermentation of Pseudomonas sp. MZ01, degraded Phe in the soil with an efficiency of up to 65.1 % at the anode, 49.9 % at the cathode after 5 days of the treatment. The presence of biosurfactants, electricity, and a neutral electrolyte stimulated the growth of the degrading bacteria as shown by a rapid increase in microbial biomass with time. The electrical conductivity and pH changed little during the course of the treatment, which benefitted the growth of microorganisms and the remediation of Phe-contaminated soil. The EMR system with the addition of biosurfactant had the highest Phe removal, demonstrating the biosurfactant may enhance the bioavailability of Phe and the interaction with the microorganism. This study suggests that the EMR combined with biosurfactants can be used to enhance in situ bioremediation of PAH-contaminated soils.

KEYWORDS:

Biosurfactants; Degradation; Electrokinetics; Microorganism; Phenanthrene

PMID:
26683200
DOI:
10.1007/s12010-015-1949-8
[Indexed for MEDLINE]

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