Format

Send to

Choose Destination
Sci Rep. 2015 Dec 18;5:18483. doi: 10.1038/srep18483.

Compact Wireless Microscope for In-Situ Time Course Study of Large Scale Cell Dynamics within an Incubator.

Author information

1
Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.
2
Department of Bioengineering, University of California, Los Angeles, Los Angeles, 90095, U.S.A.
3
School of Life Sciences, Tsinghua University, Beijing, 100084, China.
4
School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.
5
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
6
Mechanical and Aerospace Engineering Department, University of California, Los Angeles, Los Angeles, 90095, U.S.A.
7
School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, 430074, China.

Abstract

Imaging of live cells in a region of interest is essential to life science research. Unlike the traditional way that mounts CO2 incubator onto a bulky microscope for observation, here we propose a wireless microscope (termed w-SCOPE) that is based on the "microscope-in-incubator" concept and can be easily housed into a standard CO2 incubator for prolonged on-site observation of the cells. The w-SCOPE is capable of tunable magnification, remote control and wireless image transmission. At the same time, it is compact, measuring only ~10 cm in each dimension, and cost-effective. With the enhancement of compressive sensing computation, the acquired images can achieve a wide field of view (FOV) of ~113 mm(2) as well as a cellular resolution of ~3 μm, which enables various forms of follow-up image-based cell analysis. We performed 12 hours time-lapse study on paclitaxel-treated MCF-7 and HEK293T cell lines using w-SCOPE. The analytic results, such as the calculated viability and therapeutic window, from our device were validated by standard cell detection assays and imaging-based cytometer. In addition to those end-point detection methods, w-SCOPE further uncovered the time course of the cell's response to the drug treatment over the whole period of drug exposure.

PMID:
26681552
PMCID:
PMC4683435
DOI:
10.1038/srep18483
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center