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BMC Cancer. 2015 Dec 18;15:985. doi: 10.1186/s12885-015-1995-1.

Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry.

Author information

1
Division of Infectious Diseases, Federal University of Sao Paulo, 781 Pedro de Toledo Street, 15th floor, Sao Paulo, SP, 04039032, Brazil. anampassos@gmail.com.
2
Fleury Group, Sao Paulo, SP, Brazil. valdemir.carvalho@grupofleury.com.br.
3
Fleury Group, Sao Paulo, SP, Brazil. karina.cardozo@grupofleury.com.br.
4
Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil. lu1912@gmail.com.
5
Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil. alinechagas@gmail.com.
6
Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil. gomesmic@yahoo.com.br.
7
Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil. femalta@yahoo.com.
8
Department of Infectious Diseases, University of São Paulo School of Medicine, Sao Paulo, SP, Brazil. ana.cssn@gmail.com.
9
Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil. jrrpinho@usp.br.
10
Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil. fjcarril@usp.br.
11
Division of Infectious Diseases, Federal University of Sao Paulo, 781 Pedro de Toledo Street, 15th floor, Sao Paulo, SP, 04039032, Brazil. celso.granato@grupofleury.com.br.
12
Fleury Group, Sao Paulo, SP, Brazil. celso.granato@grupofleury.com.br.

Abstract

BACKGROUND:

Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers.

METHODS:

An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.

RESULTS:

The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.

CONCLUSIONS:

UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population.

PMID:
26680993
PMCID:
PMC4683945
DOI:
10.1186/s12885-015-1995-1
[Indexed for MEDLINE]
Free PMC Article

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