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PLoS One. 2015 Dec 17;10(12):e0145287. doi: 10.1371/journal.pone.0145287. eCollection 2015.

KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light.

Author information

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
2
Faculty of Biology, Moscow State University, Moscow, Russia.
3
Faculty of Medicine, Moscow State University, Moscow, Russia.
4
Pirogov Russian National Research Medical University, Moscow, Russia.
5
Laser Biospectroscopy Laboratory, Prokhorov General Physics Institute, Moscow, Russia.
6
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, 30332, United States of America.
7
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.

Abstract

Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.

PMID:
26679300
PMCID:
PMC4683004
DOI:
10.1371/journal.pone.0145287
[Indexed for MEDLINE]
Free PMC Article

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