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Nat Commun. 2015 Dec 18;6:10148. doi: 10.1038/ncomms10148.

Embryonic transcription is controlled by maternally defined chromatin state.

Author information

1
Department of Molecular Developmental Biology, Radboud Institute for Molecular Life Sciences, Faculty of Science, Radboud University, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
2
ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, Perth, Western Australia 6009, Australia.
3
The Harry Perkins Institute of Medical Research, Perth, Western Australia 6009, Australia.

Abstract

Histone-modifying enzymes are required for cell identity and lineage commitment, however little is known about the regulatory origins of the epigenome during embryonic development. Here we generate a comprehensive set of epigenome reference maps, which we use to determine the extent to which maternal factors shape chromatin state in Xenopus embryos. Using α-amanitin to inhibit zygotic transcription, we find that the majority of H3K4me3- and H3K27me3-enriched regions form a maternally defined epigenetic regulatory space with an underlying logic of hypomethylated islands. This maternal regulatory space extends to a substantial proportion of neurula stage-activated promoters. In contrast, p300 recruitment to distal regulatory regions requires embryonic transcription at most loci. The results show that H3K4me3 and H3K27me3 are part of a regulatory space that exerts an extended maternal control well into post-gastrulation development, and highlight the combinatorial action of maternal and zygotic factors through proximal and distal regulatory sequences.

PMID:
26679111
PMCID:
PMC4703837
DOI:
10.1038/ncomms10148
[Indexed for MEDLINE]
Free PMC Article

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