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Science. 2016 Jan 1;351(6268):77-81. doi: 10.1126/science.aad1283. Epub 2015 Dec 17.

An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels.

Author information

1
Department of Viroscience, Erasmus Medical Center, Rotterdam, Netherlands. b.haagmans@erasmusmc.nl gerd.sutter@lmu.de albert.osterhaus@tiho-hannover.de.
2
Department of Viroscience, Erasmus Medical Center, Rotterdam, Netherlands.
3
German Centre for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-Universität München, Munich, Germany.
4
Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
5
Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal [CReSA, IRTA-Universitat Autònoma de Barcelona (UAB)], Campus de la UAB, 08193 Bellaterra, Spain.
6
UAB, CReSA, (IRTA-UAB), Campus de la UAB, 08193 Bellaterra, Spain. Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Spain.
7
German Centre for Infection Research (DZIF), Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-Universität München, Munich, Germany. b.haagmans@erasmusmc.nl gerd.sutter@lmu.de albert.osterhaus@tiho-hannover.de.
8
Department of Viroscience, Erasmus Medical Center, Rotterdam, Netherlands. Artemis One Health, Utrecht, Netherlands. Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine, Hannover, Germany. b.haagmans@erasmusmc.nl gerd.sutter@lmu.de albert.osterhaus@tiho-hannover.de.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) infections have led to an ongoing outbreak in humans, which was fueled by multiple zoonotic MERS-CoV introductions from dromedary camels. In addition to the implementation of hygiene measures to limit further camel-to-human and human-to-human transmissions, vaccine-mediated reduction of MERS-CoV spread from the animal reservoir may be envisaged. Here we show that a modified vaccinia virus Ankara (MVA) vaccine expressing the MERS-CoV spike protein confers mucosal immunity in dromedary camels. Compared with results for control animals, we observed a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection correlated with the presence of serum neutralizing antibodies to MERS-CoV. Induction of MVA-specific antibodies that cross-neutralize camelpox virus would also provide protection against camelpox.

PMID:
26678878
DOI:
10.1126/science.aad1283
[Indexed for MEDLINE]
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