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Environ Toxicol. 2017 Jan;32(1):176-187. doi: 10.1002/tox.22224. Epub 2015 Dec 17.

Phenethyl isothiocyanate alters the gene expression and the levels of protein associated with cell cycle regulation in human glioblastoma GBM 8401 cells.

Author information

1
Division of Neurosurgical Oncology, Neurological Institute, Taichung Veterans General Hospital, Taichung, 407, Taiwan.
2
Institute of Medical Sciences, Tzu Chi University, Hualien, 970, Taiwan.
3
National Defense Medical Center, Taipei, 114, Taiwan.
4
Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, 404, Taiwan.
5
Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, 970, Taiwan.
6
Departments of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan.
7
School of Pharmacy, China Medical University, Taichung, 404, Taiwan.
8
Department of Neurosurgery, Buddhist Tzu Chi General Hospital and College of Medicine, Tzu Chi University, Hualien, 970, Taiwan.
9
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 114, Taiwan.
10
Division of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, 407, Taiwan.
11
Department of Biotechnology, Asia University, Taichung, 413, Taiwan, Republic of China.

Abstract

Glioblastoma is the most common and aggressive primary brain malignancy. Phenethyl isothiocyanate (PEITC), a member of the isothiocyanate family, can induce apoptosis in many human cancer cells. Our previous study disclosed that PEITC induces apoptosis through the extrinsic pathway, dysfunction of mitochondria, reactive oxygen species (ROS)-induced endoplasmic reticulum (ER) stress, and intrinsic (mitochondrial) pathway in human brain glioblastoma multiforme (GBM) 8401 cells. To the best of our knowledge, we first investigated the effects of PEITC on the genetic levels of GBM 8401 cells in vitro. PEITC may induce G0/G1 cell-cycle arrest through affecting the proteins such as cdk2, cyclin E, and p21 in GBM 8401 cells. Many genes associated with cell-cycle regulation of GBM 8401 cells were changed after PEITC treatment: 48 genes were upregulated and 118 were downregulated. The cell-division cycle protein 20 (CDC20), Budding uninhibited by benzimidazole 1 homolog beta (BUB1B), and cyclin B1 were downregulated, and clusterin was upregulated in GBM 8401 cells treated with PEITC. These changes of gene expression can provide the effects of PEITC on the genetic levels and potential biomarkers for glioblastoma.

KEYWORDS:

cell-cycle arrest; gene; glioblastoma; phenethyl isothiocyanate; regulation

PMID:
26678675
DOI:
10.1002/tox.22224
[Indexed for MEDLINE]

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