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Plant Pathol J. 2015 Dec;31(4):438-40. doi: 10.5423/PPJ.NT.06.2015.0110. Epub 2015 Dec 30.

Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants.

Author information

1
Water Supply and Sewerage Research Division, National Institute of Environmental Research, Incheon 404-708, Korea.
2
Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Korea.
3
Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Korea ; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, Korea.

Abstract

We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.

KEYWORDS:

LAMP; WSMV; loop-mediated isothermal amplification; quarantine; wheat streak mosaic virus

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