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Ethn Dis. 2015 Nov 5;25(4):413-8. doi: 10.18865/ed.25.4.413.

Effect of Interleukin-10 and Laminar Shear Stress on Endothelial Nitric Oxide Synthase and Nitric Oxide in African American Human Umbilical Vein Endothelial Cells.

Author information

1
1. Cedar Crest College, Allentown, Pennsylvania.
2
2. Department of Kinesiology, Temple University.
3
3. Vascular Health Laboratory, Department of Kinesiology & Nutrition, University of Illinois at Chicago.
4
2. Department of Kinesiology, Temple University ; 4.Cardiovascular Research Center, Temple University School of Medicine.

Abstract

BACKGROUND:

African Americans have a predisposition to heightened systemic inflammation and a high prevalence of hypertension.

OBJECTIVE:

The purpose of this study was to evaluate the influence of interleukin-10 (IL-10) and laminar shear stress (LSS) on African American endothelial cells by measuring total endothelial nitric oxide synthase (eNOS) protein expression and its phosphorylated form (p-eNOS) at Serine 1177, and nitric oxide (NO) levels, in response to IL-10 incubation and high physiological levels of LSS, used as an in vitro mimetic for aerobic exercise training (AEXT).

DESIGN:

Human umbilical vein endothelial cells (HUVEC) from an African American donor were cultured. The experimental conditions included Static, Static with IL-10 Incubation, LSS at 20 dynes/cm², and LSS at 20 dynes/cm² with IL-10 Incubation. Western blotting was used to measure eNOS and p-eNOS protein expression in the cells. A modified Griess assay was used to measure NO metabolites in the cell culture media.

RESULTS:

There were significant increases in p-eNOS, eNOS, and NO in the LSS at 20 dynes/cm² and LSS at 20 dynes/cm² with IL-10 Incubation experimental conditions when compared to the Static experimental condition. There were no other statistically significant differences demonstrating that IL-10 did not have an additive effect on eNOS activity in our study.

CONCLUSION:

The significant increases in p-eNOS, eNOS, and NO as a result of LSS in African American HUVECs suggest that AEXT may be a viable, nonpharmacologic method to improve vascular inflammation status and vasodilation, and thereby contribute to hypertension reduction in the African American population.

KEYWORDS:

African Americans; Endothelial Cells; Endothelial Dysfunction; Endothelial Nitric Oxide Synthase; Inflammation; Interleukin-10; Laminar Shear Stress; Nitric Oxide

PMID:
26674844
PMCID:
PMC4671427
DOI:
10.18865/ed.25.4.413
[Indexed for MEDLINE]
Free PMC Article

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