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J Environ Sci Health B. 2016;51(3):176-81. doi: 10.1080/03601234.2015.1108811. Epub 2015 Dec 16.

Biologically active antimicrobial and antioxidant substances in the Helianthus annuus L. bee pollen.

Author information

1
a Faculty of Agrobiology and Food Resources , Department of Human Nutrition, Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
2
b Faculty of Agrobiology and Food Resources , Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
3
c Allvit , Bratislava , Slovak Republic.
4
d Faculty of Biotechnology and Food Science , Department of Microbiology, Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.

Abstract

The objective of this study was to measure the content of flavonoids, polyphenols, and carotenoids in the Helianthus annuus L. bee pollen. It was also to evaluate the ability of the dried, frozen, and freeze-dried extracts of sunflower (H. annuus) pollen, its scavenged free radicals and reducing action. Another aim of this study was to investigate the antimicrobial in vitro action of the H. annuus pollen extracts against the Gram-positive and Gram-negative bacteria and fungi. All pollen extracts showed medium antiradical activity and reductive ability. The most effective was the freeze-dried extract in both evaluation systems. The evaluation of the protective effects of DNA using a biosensor showed an opposite trending-frozen ˃ dried ˃ freeze-dried pollen. For the evaluation of antiradical activity, the DPPH method was used, and reductive ability was assessed by means of phosphomolybdic complex formation. The comparison of the polyphenols content shows higher values in freeze-dried bee pollen than in the dried and frozen pollen. The highest content of flavonoids was found in the frozen samples and the most carotenoids were present in the dried samples. In our study, the best antibacterial effects of the dried sunflower bee pollen extracts were found against Paenibacillus larvae, Pseudomonas aeruginosa, and Enterococcus raffinosus. The best inhibitory properties of the frozen sunflower bee pollen extracts were found against Escherichia coli, Pseudomonas aeruginosa, and Paenibacillus larvae. Very good inhibitory effects of freeze-dried sunflower bee pollen were found against Paenibacillus larvae, Brochotrix thermosphacta, and Enterococcus raffinosus. The best antifungal activity of the sunflower bee pollen was found in the frozen bee pollen extracts against Aspergillus ochraceus and freeze-dried bee pollen extracts against Aspergillus niger.

KEYWORDS:

Bee pollen; Helianthus annuus L; antimicrobial action; antioxidant activity; flavonoids

PMID:
26674447
DOI:
10.1080/03601234.2015.1108811
[Indexed for MEDLINE]

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